About

Ageing with elegans is a project funded by the European Commission under the Horizon 2020 Research and Innovation programme based on a collaboration of thirteen academic research groups and four companies from thirteen EU and three non-EU countries (Switzerland, USA, and China). The project started on May 1st 2015 and will run until April 31st 2020. The consortium will validate C. elegans as a healthspan model for better understanding of the factors responsible for healthy ageing, and will develop evidence-based prevention, diagnostic, and therapeutic strategies.

Problem statement:

Healthspan (the life period when one is generally healthy and free from serious disease) depends on nature (genetic make-up) and nurture (environmental influences, from the earliest stages of development throughout life). Genetic studies increasingly reveal mutations and polymorphisms that may affect healthspan. Similarly, claims abound about lifestyle modifications or treatments improving healthspan. In both cases, rigorous testing is hampered by the long lifespan of model organisms like mice (let alone humans) and the difficulty of introducing genetic changes to examine the phenotype of the altered genome.

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Copyright. Ineke Dhondt (UGent)

Solution:

This project will develop concepts and methods  for using C. elegans as a healthspan model. Already validated extensively as an ageing model, this organism can be readily modified genetically, and effects of environmental manipulations on healthspan can be measured in days or weeks. Once validated as a healthspan model, it can be used for an initial assessment of preventive and therapeutic measures for humans, as well as for risk identification and the initial evaluation of potential biomarkers. It will also prove useful to study interactions between genetic and various environmental factors.

Approach:

A high-throughput assay for healthspan in C. elegans will be used to conduct a genome-wide RNAi screen for genes affecting healthspan. These genes will be compared with the ones showing under- or over-represented polymorphisms in cohorts of elderly persons who aged exceptionally well. Moreover, the molecular targets of medicinal plants that increase healthspan in C. elegans as well as in mice will be elucidated both in C. elegans as well as mammalian cell systems. In addition, intervention testing as well as target and pathway discovery will include approaches towards identifying best synergistic combinations. From these combined human, C. elegans, mouse and mammalian cell data, molecular pathways affecting healthspan will be derived using (amongst others) advanced data mining methods that involve state-of the-art semantic approaches and network biology tools.

Partner Institutes

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