WP5 – Mammalian cellular mode

Validation for health-preserving and health-promoting effects of natural and synthetic compounds using the experimental system of long term culturing and serial passaging of normal and immortalized human cells, especially skin fibroblasts, vascular endothelial cells and telomerase-immortalized bone marrow stem cells. Cell type-specific functional assays include migration and wound healing for fibroblasts, angiogenesis for endothelial cells, and osteoblastic differentiation for bone marrow stem cells. Along with the standard lifespan assays, novel healthspan assays will be developed for measuring the health status of human cells in terms of overall stress resistance, resilience and robustness.